How Long Does It Last? When dried completely, and then stored properly, a sourdough starter can last indefinitely at room temperature. But, freezing can give you extra piece of mind. So, here are some things to consider when thinking about long term storage. In the video linked above, I show all the steps involved, and talk about the differences between reactivating frozen vs room temperature dried fresh milled flour sourdough starter. *(See full post for storage tips, and what to avoid and look for.)